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glosensor-22f luminescent camp-sensing protein  (Promega)

 
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    Structured Review

    Promega glosensor-22f luminescent camp-sensing protein
    Glosensor 22f Luminescent Camp Sensing Protein, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor-22f+luminescent+camp-sensing+protein/glosensor+22f+luminescent+camp+sensing+protein/pm38091482-125-9-6
    Average 90 stars, based on 1 article reviews
    glosensor-22f luminescent camp-sensing protein - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein48 were grown to confluence and collected, and then one-third of the collected cells was plated onto a 10-cm tissue-treated dish with 10 ml of 10% FBS in DMEM without penicillin/streptomycin. ..

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein were collected, transfected and transferred to 96-well plates identically as above (cAMP activation assay), except 1.2 μg of NLuc-fused GLP-1R plasmid (NLuc-GLP-1R as a solution in endotoxin-free TE buffer) and 5 μl of FuGENE HD reagent were used for the transfection step. ..

    Article Title: Effects of Replacing a Central Glycine Residue in GLP-1 on Receptor Affinity and Signaling Profile.
    Article Snippet: .. The protocol was adapted from Hager et al.[10] and Binkowski et al.[31] HEK293 cells stably expressing Glosensor-22F (Promega) luminescent cAMP-sensing protein were seeded at a density of ~2.5 million cells on a 10 cm plate. ..

    Article Title: Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS).
    Article Snippet: The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.. Signal transduction processes mediated by GPCR activation typically result in receptor phosphorylation, but very little is known about GIPR phosphorylation.. Mass spectrometry (MS) is a powerful tool for detecting phosphorylation and other post-translational modifications of proteins and for identifying modification sites.

    Expressing:

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein48 were grown to confluence and collected, and then one-third of the collected cells was plated onto a 10-cm tissue-treated dish with 10 ml of 10% FBS in DMEM without penicillin/streptomycin. ..

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein were collected, transfected and transferred to 96-well plates identically as above (cAMP activation assay), except 1.2 μg of NLuc-fused GLP-1R plasmid (NLuc-GLP-1R as a solution in endotoxin-free TE buffer) and 5 μl of FuGENE HD reagent were used for the transfection step. ..

    Article Title: Effects of Replacing a Central Glycine Residue in GLP-1 on Receptor Affinity and Signaling Profile.
    Article Snippet: .. The protocol was adapted from Hager et al.[10] and Binkowski et al.[31] HEK293 cells stably expressing Glosensor-22F (Promega) luminescent cAMP-sensing protein were seeded at a density of ~2.5 million cells on a 10 cm plate. ..

    Article Title: Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS).
    Article Snippet: The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.. Signal transduction processes mediated by GPCR activation typically result in receptor phosphorylation, but very little is known about GIPR phosphorylation.. Mass spectrometry (MS) is a powerful tool for detecting phosphorylation and other post-translational modifications of proteins and for identifying modification sites.

    Transfection:

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein were collected, transfected and transferred to 96-well plates identically as above (cAMP activation assay), except 1.2 μg of NLuc-fused GLP-1R plasmid (NLuc-GLP-1R as a solution in endotoxin-free TE buffer) and 5 μl of FuGENE HD reagent were used for the transfection step. ..

    Article Title: Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS).
    Article Snippet: The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.. Signal transduction processes mediated by GPCR activation typically result in receptor phosphorylation, but very little is known about GIPR phosphorylation.. Mass spectrometry (MS) is a powerful tool for detecting phosphorylation and other post-translational modifications of proteins and for identifying modification sites.

    Activation Assay:

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein were collected, transfected and transferred to 96-well plates identically as above (cAMP activation assay), except 1.2 μg of NLuc-fused GLP-1R plasmid (NLuc-GLP-1R as a solution in endotoxin-free TE buffer) and 5 μl of FuGENE HD reagent were used for the transfection step. ..

    Plasmid Preparation:

    Article Title: Structural and functional diversity among agonist-bound states of the GLP-1 receptor.
    Article Snippet: .. HEK293 cells stably expressing the GloSensor-22F (Promega) luminescent cAMP-sensing protein were collected, transfected and transferred to 96-well plates identically as above (cAMP activation assay), except 1.2 μg of NLuc-fused GLP-1R plasmid (NLuc-GLP-1R as a solution in endotoxin-free TE buffer) and 5 μl of FuGENE HD reagent were used for the transfection step. ..

    Synthesized:

    Article Title: Impact of substitution registry on receptor-activation profiles of backbone-modified glucagon-like peptide-1 analogues
    Article Snippet: .. Peptide Synthesis: Peptides were synthesized by standard solid-phase methods using NovaPEG rink amide resin.. cAMP-Production: Protocol was adapted from Hager et al. and Binkowski et al. HEK293 cells stably expressing the Glosensor-22F (Promega) luminescent cAMP-sensing protein were grown to confluence and then plated 1:3 onto a 10 cm tissue-treated dish with 10 mL of appropriate culture medium ( vide supra ) without penicillin/streptomycin. ..



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    glosensor-22f luminescent camp-sensing protein  (Promega)
    90
    Promega glosensor-22f luminescent camp-sensing protein
    Glosensor 22f Luminescent Camp Sensing Protein, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor-22f+luminescent+camp-sensing+protein/glosensor+22f+luminescent+camp+sensing+protein/pm38091482-125-9-6
    Average 90 stars, based on 1 article reviews
    glosensor-22f luminescent camp-sensing protein - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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